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Anti-PTPLB Polyclonal Antibody

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Catalog No. TMAB-11919 Copy Product Info
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Anti-PTPLB Polyclonal Antibody is a Rabbit antibody targeting PTPLB. Anti-PTPLB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PTPLB Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2227-10 days7-10 days
100 µL$3747-10 days7-10 days
200 µL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PTPLB Polyclonal Antibody is a Rabbit antibody targeting PTPLB. Anti-PTPLB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Sample: DU145 (Human) Cell Lysate at 30 μg
Primary: Anti-PTPLB (TMAB-11919) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
2. Sample: LOVo (Human) Cell Lysate at 30 μg
Primary: Anti-PTPLB (TMAB-11919) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PTPLB) Polyclonal Antibody, Unconjugated (TMAB-11919) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane.
Tissue SpecificityHighly expressed in testis, spleen, prostate, colon and heart, followed by moderate expression in thymus, ovary, small intestine, peripheral blood leukocytes, liver, skeletal muscle and pancreas. Weakly detected in kidney, placenta, brain and lung.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene can catalyze the third step (dehydration) in the conversion of long chain fatty acids to very long chain fatty acids. The encoded protein localizes to the endoplasmic reticulum membrane. [provided by RefSeq, Jul 2016]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PTPLB
Antigen Species
Human
Gene Name
HACD2
Gene ID
Protein Name
Very-long-chain(3R)-3-hydroxyacyl-CoA dehydratase 2
Uniprot ID
Function
Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis.
Chemical Properties
Molecular WeightTheoretical: 28 kDa. Actual: 28 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-PTPLB Polyclonal Antibody molecular weight