Anti-PSME1 Antibody (7S197) is a Rabbit antibody targeting PSME1. Anti-PSME1 Antibody (7S197) can be used in WB,ICC/IF,IP,FCM.

IgG

7S197

Human,Mouse,Rat

1. Western blot analysis of Jurkat cell lysate. Using PSME1 (TMAB-11878) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PSME1 antibody (TMAB-11878, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human PSME1

Human

PSME1

Proteasome activator complex subunit 1

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.