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Anti-PSGL-1/CD162 Antibody (3U811)
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Catalog No. TMAY-02646
Alias selectin P ligand, PSGL-1, PSGL1, CLA, CD162
Anti-PSGL-1/CD162 Antibody (3U811) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (3U811) can be used in IHC-P,ICC/IF.
Anti-PSGL-1/CD162 Antibody (3U811)
Anti-PSGL-1/CD162 Antibody (3U811)
😃Good
Catalog No. TMAY-02646Alias selectin P ligand, PSGL-1, PSGL1, CLA, CD162
Anti-PSGL-1/CD162 Antibody (3U811) is a Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (3U811) can be used in IHC-P,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Mouse Monoclonal Application: IHC-P, ICC/IF Reactivity: Human |
| Alias | selectin P ligand, PSGL-1, PSGL1, CLA, CD162 |
| Ig Type | Mouse IgG1 |
| Clone | 3U811 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human CD162 in human spleen with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 2. Immunochemical staining of human CD162 in human tonsil with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 3. Immunofluorescence staining of SELPLG- in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human SELPLG- monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| Application | IHC-P,ICC/IF |
| Recommended Dose | IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PSGL-1/CD162 Protein (TMPY-03531) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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