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Anti-PRSS8 Polyclonal Antibody
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Catalog No. TMAB-11839
Anti-PRSS8 Polyclonal Antibody is a Rabbit antibody targeting PRSS8. Anti-PRSS8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PRSS8 Polyclonal Antibody
Anti-PRSS8 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11839
Anti-PRSS8 Polyclonal Antibody is a Rabbit antibody targeting PRSS8. Anti-PRSS8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-PRSS8 Polyclonal Antibody is a Rabbit antibody targeting PRSS8. Anti-PRSS8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | 1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-PRSS8 Polyclonal Antibody, Unconjugated (TMAB-11839) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-PRSS8 Polyclonal Antibody, Unconjugated (TMAB-11839) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Sample: Lane 1: Muscle (Mouse) Lysate at 40 μg Lane 2: Muscle (Rat) Lysate at 40 μg Primary: Anti-PRSS8 (TMAB-11839) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37-40 kD Observed band size: 40 kD 4. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-PRSS8 (TMAB-11839) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30/33 kD Observed band size: 40/43 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Prostasin: Cell membrane; Single-pass membrane protein. Prostasin light chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus. Prostasin heavy chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus. |
| Tissue Specificity | Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | PRSS8 is a member of the trypsin family of serine proteases. It can be found in seminal fluid and is highly expressed in prostate epithelia. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. The carboxyterminal region contains a GPI anchor sequence, which anchors prostasin to the cell surface. Phospholipase activity is thought to liberate prostasin from the cell, and cleavage of the linker region also releases prostasin. PRSS8 has been proposed as a marker for ovarian cancer, where it is up-regulated, a marker for androgen-refractory prostate cancer, where it is down-regulated, and as an inhibitor of invasiveness in breast cancer. |
| Immunogen | KLH conjugated synthetic peptide: human PRSS8 |
| Antigen Species | Human |
| Gene Name | PRSS8 |
| Gene ID | |
| Protein Name | Prostasin |
| Uniprot ID | |
| Function | Possesses a trypsin-like cleavage specificity. |
| Molecular Weight | Theoretical: 30/33 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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