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Anti-PRP18 homolog Polyclonal Antibody

Catalog No. TMAB-11815 Copy Product Info
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Anti-PRP18 homolog Polyclonal Antibody is a Rabbit antibody targeting PRP18 homolog. Anti-PRP18 homolog Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PRP18 homolog Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-11815

Anti-PRP18 homolog Polyclonal Antibody is a Rabbit antibody targeting PRP18 homolog. Anti-PRP18 homolog Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PRP18 homolog Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PRP18 homolog Polyclonal Antibody is a Rabbit antibody targeting PRP18 homolog. Anti-PRP18 homolog Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PRP18 homolog) Polyclonal Antibody, Unconjugated (TMAB-11815) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-PRP18 homolog (TMAB-11815) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus speckle. Colocalizes with spliceosomal snRNPs.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PRP18 homolog
Antigen Species
Human
Gene Name
PRPF18
Gene ID
Protein Name
Pre-mRNA-splicing factor 18
Uniprot ID
Function
Participates in the second step of pre-mRNA splicing.
Chemical Properties
Molecular WeightTheoretical: 40 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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