Anti-Prostatic Acid Phosphatase Polyclonal Antibody is a Rabbit antibody targeting Prostatic Acid Phosphatase. Anti-Prostatic Acid Phosphatase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Horse)

1. Sample: PC3 Cell (Human) Lysate at 40 μg
Primary: Anti-Prostatic Acid Phosphatase (TMAB-11798) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 48 kD
2. Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Prostatic Acid Phosphatase) Polyclonal Antibody, Unconjugated (TMAB-11798) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Prostatic Acid Phosphatase

Human

ACP3

Prostatic acid phosphatase

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.