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Anti-Progesterone Receptor Polyclonal Antibody

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Catalog No. TMAB-01578
Alias Progestin receptor form B, Progestin receptor form A, Progesterone receptor, PRGR, PRB, PRA, PR, PGR, Nuclear receptor subfamily 3 group C member 3, NR3C3

Anti-Progesterone Receptor Polyclonal Antibody is a Rabbit antibody targeting Progesterone Receptor. Anti-Progesterone Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Progesterone Receptor Polyclonal Antibody

Anti-Progesterone Receptor Polyclonal Antibody

😃Good
Catalog No. TMAB-01578Alias Progestin receptor form B, Progestin receptor form A, Progesterone receptor, PRGR, PRB, PRA, PR, PGR, Nuclear receptor subfamily 3 group C member 3, NR3C3
Anti-Progesterone Receptor Polyclonal Antibody is a Rabbit antibody targeting Progesterone Receptor. Anti-Progesterone Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Progesterone Receptor Polyclonal Antibody is a Rabbit antibody targeting Progesterone Receptor. Anti-Progesterone Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasProgestin receptor form B, Progestin receptor form A, Progesterone receptor, PRGR, PRB, PRA, PR, PGR, Nuclear receptor subfamily 3 group C member 3, NR3C3
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse uterus tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PRGR) Polyclonal Antibody, Unconjugated (TMAB-01578) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Progesterone Receptor) Polyclonal Antibody, Unconjugated (TMAB-01578) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Progesterone Receptor antibody (TMAB-01578)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Progesterone Receptor) Polyclonal Antibody, Unconjugated (TMAB-01578) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. 25 μg total protein per Lane of various lysates probed with Progesterone Receptor polyclonal antibody, unconjugated (TMAB-01578) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases. Isoform A: Nucleus. Cytoplasm. Note=Mainly nuclear.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundEstrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER alpha and ER beta. ER alpha and ER beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-Aand hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Progesterone Receptor
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Progesterone,Receptors,Progesterone,Zinc Finger,Gonadotrophic axis,Progesterone
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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