Anti-Procalcitonin (PCT) Antibody (7R665) is a Mouse antibody targeting Procalcitonin (PCT). Anti-Procalcitonin (PCT) Antibody (7R665) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

7R665

Human

1. Sample:
Lane 1: Recombinant human Procalcitonin, N-His
Primary: Anti-Procalcitonin (PCT) (TMAB-11763) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Procalcitonin (PCT)) Monoclonal Antibody, Unconjugated (TMAB-11763) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Procalcitonin (PCT) Monoclonal Antibody, Unconjugated (TMAB-11763) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, BF488), DAPI (blue) was used to stain the cell nuclei.
4. 4% Paraformaldehyde-fixed TT (treated with BFA) (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Procalcitonin(PCT)) monoclonal Antibody, unconjugated (TMAB-11763) 1: 100, 90 min at 37°C; followed by FITC conjugated Goat Anti-Mouse IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

KLH conjugated synthetic peptide: human Procalcitonin(PCT)

Human

CALCA

Calcitonin

The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a proinflammatory stimulus, especially of bacterial origin. It does not rise significantly with viral or non-infectious inflammations. With the derangements that a severe infection with an associated systemic response brings, the blood levels of procalcitonin may rise to 100 µg/L. In serum, procalcitonin has a half-life of 25 to 30 hours. Remarkably the high procalcitonin levels produced during infections are not followed by a parallel increase in calcitonin or a decrease in serum calcium levels.