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Anti-PRKCD Antibody (8E672)
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Catalog No. TMAC-03316
Alias Tyrosine-protein kinase PRKCD, Protein kinase C delta type, PRKCD, PKCD, nPKC-delta
Anti-PRKCD Antibody (8E672) is a Rabbit antibody targeting PRKCD. Anti-PRKCD Antibody (8E672) can be used in WB,ICC/IF,IHC.
Anti-PRKCD Antibody (8E672)
Anti-PRKCD Antibody (8E672)
😃Good
Catalog No. TMAC-03316Alias Tyrosine-protein kinase PRKCD, Protein kinase C delta type, PRKCD, PKCD, nPKC-delta
Anti-PRKCD Antibody (8E672) is a Rabbit antibody targeting PRKCD. Anti-PRKCD Antibody (8E672) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-PRKCD Antibody (8E672) is a Rabbit antibody targeting PRKCD. Anti-PRKCD Antibody (8E672) can be used in WB,ICC/IF,IHC. |
| Synonyms | Tyrosine-protein kinase PRKCD, Protein kinase C delta type, PRKCD, PKCD, nPKC-delta |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin. 8. ICC staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining PKC delta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining PKC delta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. ICC staining PKC delta in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 78 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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