Anti-PREPL Antibody (8F958) is a Rabbit antibody targeting PREPL. Anti-PREPL Antibody (8F958) can be used in ICC/IF,IP,FCM,IHC-P.

IgG

8F958

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PREPL Monoclonal Antibody, Unconjugated (TMAB-11724) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PREPL Monoclonal Antibody, Unconjugated (TMAB-11724) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PREPL Monoclonal Antibody, Unconjugated (TMAB-11724) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PREPL antibody (TMAB-11724, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100; IHC-P: 1:50-200

Monoclonal

A synthesized peptide: human PREPL

Human

PREPL

Prolyl endopeptidase-like