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Anti-PRDM1 Antibody (5J185)

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Catalog No. TMAB-11715

Anti-PRDM1 Antibody (5J185) is a Mouse antibody targeting PRDM1. Anti-PRDM1 Antibody (5J185) can be used in WB,ICC/IF.

Anti-PRDM1 Antibody (5J185)

Anti-PRDM1 Antibody (5J185)

Copy Product Info
😃Good
Catalog No. TMAB-11715
Anti-PRDM1 Antibody (5J185) is a Mouse antibody targeting PRDM1. Anti-PRDM1 Antibody (5J185) can be used in WB,ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-PRDM1 Antibody (5J185) is a Mouse antibody targeting PRDM1. Anti-PRDM1 Antibody (5J185) can be used in WB,ICC/IF.
Ig Type
IgG1, Kappa
Clone
5J185
Reactivity
Human
Verified Activity
1. Cell line: Ramos
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-11715
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Lysate: Raji, Daudi, Ramos, THP-1
Protein loading quantity: 20 μg
Exposure time: 180 s
Predicted MW: 90 kDa
Observed MW: 90 kDa
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-100
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNucleus. Cytoplasm.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]
Antigen Details
Gene Name
PRDM1
Gene ID
Protein Name
PR domain zinc finger protein 1
Function
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells.
Chemical Properties
Molecular WeightTheoretical: 92 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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