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Anti-PRCP Polyclonal Antibody 2
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Catalog No. TMAB-11712
Anti-PRCP Polyclonal Antibody 2 is a Rabbit antibody targeting PRCP. Anti-PRCP Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PRCP Polyclonal Antibody 2
Anti-PRCP Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-11712
Anti-PRCP Polyclonal Antibody 2 is a Rabbit antibody targeting PRCP. Anti-PRCP Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-PRCP Polyclonal Antibody 2 is a Rabbit antibody targeting PRCP. Anti-PRCP Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Dog,Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PRCP) Polyclonal Antibody, Unconjugated (TMAB-11712) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. 2. Sample: bone (Mouse) Lysate at 40 μg Primary: Anti-PRCP(TMAB-11712) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lysosome. |
| Tissue Specificity | Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | PRCP belongs to the peptidase S28 family. It cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. |
| Immunogen | KLH conjugated synthetic peptide: human PRCP |
| Antigen Species | Human |
| Gene Name | PRCP |
| Gene ID | |
| Protein Name | Lysosomal Pro-X carboxypeptidase |
| Uniprot ID | |
| Function | Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. |
| Molecular Weight | Theoretical: 55 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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