Anti-PPP3CB Antibody (8F182) is a Mouse antibody targeting PPP3CB. Anti-PPP3CB Antibody (8F182) can be used in WB, IHC-P, IHC-Fr, IF.

IgG1, Kappa

8F182

Human,Mouse (predicted:Rat)

1. Tissue: Human brain
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-11693
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 59 kDa
Observed MW: 59 kDa

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

PPP3CB

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.