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Anti-PPP3CA Polyclonal Antibody 2
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Catalog No. TMAB-11690
Anti-PPP3CA Polyclonal Antibody 2 is a Mouse antibody targeting PPP3CA. Anti-PPP3CA Polyclonal Antibody 2 can be used in FCM.
Anti-PPP3CA Polyclonal Antibody 2
Anti-PPP3CA Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-11690
Anti-PPP3CA Polyclonal Antibody 2 is a Mouse antibody targeting PPP3CA. Anti-PPP3CA Polyclonal Antibody 2 can be used in FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-PPP3CA Polyclonal Antibody 2 is a Mouse antibody targeting PPP3CA. Anti-PPP3CA Polyclonal Antibody 2 can be used in FCM. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-PPP3CA antibody (TMAB-11690): 2 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 μg/test. Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. |
| Tissue Specificity | Interacts with CHP1 and CHP2. Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. Interacts with CMYA5; this interaction represses calcineurin activity in muscle. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively). |
| Immunogen | KLH conjugated synthetic peptide: human PPP3CA |
| Antigen Species | Human |
| Gene Name | PPP3CA |
| Gene ID | |
| Protein Name | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform |
| Uniprot ID | |
| Function | Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. |
| Molecular Weight | Theoretical: 57 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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