Anti-PPP2R5E Antibody (2X714) is a Rabbit antibody targeting PPP2R5E. Anti-PPP2R5E Antibody (2X714) can be used in FCM, ICC/IF, WB.

IgG

2X714

Human,Mouse,Rat

1. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PPP2R5E antibody (TMAB-11688, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 ug total protein per lane of various lysates (see on figure) probed with PPP2R5E monoclonal antibody, unconjugated (TMAB-11688) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM=1:50-100; ICC/IF=1:50-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human PPP2R5E

Human

PPP2R5E

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.