Anti-PPP2R3 Polyclonal Antibody is a Rabbit antibody targeting PPP2R3. Anti-PPP2R3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Dog,Horse,Rabbit)

1. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-PPP2R3 (TMAB-11682) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
2. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPP2R3) Polyclonal Antibody, Unconjugated (TMAB-11682) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample: Skeletal muscle (Mouse) Lysate at 40 μg
Primary: Anti-PPP2R3 (TMAB-11682) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
4. Sample: Lung (Mouse) Lysate at 40 μg
Primary: Anti-PPP2R3 (TMAB-11682) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PPP2R3

Human

PPP2R3A

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.