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Anti-PPP1R1A Antibody (5B434) is a Rabbit antibody targeting PPP1R1A. Anti-PPP1R1A Antibody (5B434) can be used in IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-PPP1R1A Antibody (5B434) is a Rabbit antibody targeting PPP1R1A. Anti-PPP1R1A Antibody (5B434) can be used in IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM. |
| Ig Type | IgG |
| Clone | 5B434 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Rat brain lysate. Using PPP1R1A (TMAB-11676) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPP1R1A Monoclonal Antibody, Unconjugated (TMAB-11676) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPP1R1A Monoclonal Antibody, Unconjugated (TMAB-11676) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPP1R1A Monoclonal Antibody, Unconjugated (TMAB-11676) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPP1R1A Monoclonal Antibody, Unconjugated (TMAB-11676) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPP1R1A Monoclonal Antibody, Unconjugated (TMAB-11676) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPP1R1A Monoclonal Antibody, Unconjugated (TMAB-11676) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; IP: 1:20-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase at Threonine 35. In addition, the proline-directed kinases Cdk1, Cdk5, and mitogen-activated protein kinase (MAPK) mediate in vitro phosphorylation of IPP-1 at the phylogenetically conserved position Serine 67. |
| Immunogen | A synthesized peptide: human PPP1R1A |
| Antigen Species | Human |
| Gene Name | PPP1R1A |
| Gene ID | |
| Protein Name | Protein phosphatase 1 regulatory subunit 1A |
| Uniprot ID |
| Molecular Weight | Theoretical: 19 kDa. Actual: 27 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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