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Anti-PPP1R1A Antibody (4O879)
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Catalog No. TMAC-03403
Alias Protein Phosphatase Inhibitor 1, Protein Phosphatase 1 Regulatory Subunit 1A, PPP1R1A, IPP-1, IPP1, I-1
Anti-PPP1R1A Antibody (4O879) is a Rabbit antibody targeting PPP1R1A. Anti-PPP1R1A Antibody (4O879) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-PPP1R1A Antibody (4O879)
Anti-PPP1R1A Antibody (4O879)
😃Good
Catalog No. TMAC-03403Alias Protein Phosphatase Inhibitor 1, Protein Phosphatase 1 Regulatory Subunit 1A, PPP1R1A, IPP-1, IPP1, I-1
Anti-PPP1R1A Antibody (4O879) is a Rabbit antibody targeting PPP1R1A. Anti-PPP1R1A Antibody (4O879) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-PPP1R1A Antibody (4O879) is a Rabbit antibody targeting PPP1R1A. Anti-PPP1R1A Antibody (4O879) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Protein Phosphatase Inhibitor 1, Protein Phosphatase 1 Regulatory Subunit 1A, PPP1R1A, IPP-1, IPP1, I-1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PPP1R1A on Rat brain lysates using anti-PPP1R1A antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PPP1R1A antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PPP1R1A antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PPP1R1A antibody. Counter stained with hematoxylin. 5. ICC staining PPP1R1A in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining PPP1R1A in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PPP1R1A in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY5Y cells with PPP1R1A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:100-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The inhibitor of protein phosphatase 1 (IPP-1, I-1) plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase. IPP-1 is present in skeletal muscles and in distinct neuronal systems of the brain. The localization and expression of IPP-1 suggests that it may play discrete roles in certain regions and developing stages of the brain, independent of the regulation of protein phosphatase type 1 (PP-1). PP-1 binds to both phosphorylated and dephosphorylated IPP-1. Conversion of PP-1 to an Mn2+-dependent state appears to play a role in its regulation by IPP-1. IPP-1 attenuates the activity of glycogen phosphorylase and is thought to play an important role in the hormonal control of glycogen metabolism. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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