Anti-PPARGC1A Polyclonal Antibody is a Rabbit antibody targeting PPARGC1A. Anti-PPARGC1A Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG

Human (predicted:Mouse,Rat,Pig,Goat)

1. Sample:
Lane 1: Recombinant human PPARGC1A, N-Trx-His
Primary: Anti-PPARGC1A (TMAB-01570) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 61 kDa
2. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PPARGC1A) polyclonal Antibody, Unconjugated (TMAB-01570) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25-100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PGC-1

Human

PPARGC1A

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha

Diabetes associated,Metabolism,Co-activators/co-repressors,Estrogen,Other factors,Energy Metabolism,Fatty acids,Mitochondrial markers,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Energy Metabolism,Mitochondrial

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.