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Anti-PPARGC1A Polyclonal Antibody

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Catalog No. TMAB-01570

Anti-PPARGC1A Polyclonal Antibody is a Rabbit antibody targeting PPARGC1A. Anti-PPARGC1A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-PPARGC1A Polyclonal Antibody

Anti-PPARGC1A Polyclonal Antibody

😃Good
Catalog No. TMAB-01570
Anti-PPARGC1A Polyclonal Antibody is a Rabbit antibody targeting PPARGC1A. Anti-PPARGC1A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PPARGC1A Polyclonal Antibody is a Rabbit antibody targeting PPARGC1A. Anti-PPARGC1A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Pig,Goat)
Verified Activity
1. Sample:
Lane 1: Recombinant human PPARGC1A, N-Trx-His
Primary: Anti-PPARGC1A (TMAB-01570) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 61 kDa
2. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PPARGC1A) polyclonal Antibody, Unconjugated (TMAB-01570) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25-100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PGC-1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Diabetes associated,Metabolism,Co-activators/co-repressors,Estrogen,Other factors,Energy Metabolism,Fatty acids,Mitochondrial markers,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Energy Metabolism,Mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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