Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Rabbit,Sheep)

IgG

Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Rabbit,Sheep)

1. Tissue/cell: rat aorta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PPAR Gamma Polyclonal Antibody, Unconjugated (TMAB-01569) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-PPAR Gamma antibody (TMAB-01569), Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test.
3. Sample:
Liver (Mouse) Lysate at 40 μg
Heart (Mouse) Lysate at 40 μg
Lung (Mouse) Lysate at 40 μg
Primary: Anti-PPAR gamma (TMAB-01569) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 51 kDa

WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PPAR Gamma

Human

5468

Diabetes associated,Metabolism,Zinc Finger,Fatty acids,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Metabolism,Adipogenesis