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Anti-PPAR gamma/PPARG Antibody (1Z782)
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Catalog No. TMAB-01567
Alias PPAR-gamma, PPARG, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3
Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in IF,IHC-Fr,IHC-P,WB.
Anti-PPAR gamma/PPARG Antibody (1Z782)
Anti-PPAR gamma/PPARG Antibody (1Z782)
😃Good
Catalog No. TMAB-01567Alias PPAR-gamma, PPARG, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3
Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | PPAR-gamma, PPARG, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3 |
| Ig Type | IgG1 |
| Clone | 1Z782 |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Eye (Mouse) Lysate at 40 μg Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa 2. Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 μg Lane 2: A431 (Human) Cell Lysate at 30 μg Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 50 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (TMAB-01567) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nuclear |
| Tissue Specificity | Skeletal muscle, liver, heart and kidney. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human PPAR gamma |
| Antigen Species | Human |
| Gene Name | PPARG |
| Gene ID | |
| Protein Name | Peroxisome proliferator-activated receptor gamma |
| Uniprot ID | |
| Biology Area | Diabetes associated,Metabolism,Zinc Finger,Fatty acids,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Metabolism,Adipogenesis |
| Function | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. |
| Molecular Weight | Theoretical: 57 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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