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Anti-PPAR gamma/PPARG Antibody (1Z782)

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Catalog No. TMAB-01567

Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PPAR gamma/PPARG Antibody (1Z782)

Anti-PPAR gamma/PPARG Antibody (1Z782)

😃Good
Catalog No. TMAB-01567
Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$373 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse (predicted:Rat)
Ig Type
IgG1
Clone
1Z782
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Sample:
Eye (Mouse) Lysate at 40 μg
Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
2. Sample:
Lane 1: Liver (Mouse) Tissue Lysate at 40 μg
Lane 2: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (TMAB-01567) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNuclear
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PPAR gamma
Antigen Species
Human
Gene ID
5468
Uniprot ID
Biology Area
Diabetes associated,Metabolism,Zinc Finger,Fatty acids,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Metabolism,Adipogenesis
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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