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Anti-PPAR gamma/PPARG Antibody (1Z782) is a Mouse antibody targeting PPAR gamma/PPARG. Anti-PPAR gamma/PPARG Antibody (1Z782) can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse (predicted:Rat) |
Ig Type | IgG1 |
Clone | 1Z782 |
Reactivity | Human,Mouse (predicted:Rat) |
Verified Activity | 1. Sample: Eye (Mouse) Lysate at 40 μg Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa 2. Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 μg Lane 2: A431 (Human) Cell Lysate at 30 μg Primary: Anti-PPAR gamma (TMAB-01567) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 50 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (TMAB-01567) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Nuclear |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
Immunogen | KLH conjugated synthetic peptide: human PPAR gamma |
Antigen Species | Human |
Gene ID | 5468 |
Uniprot ID | |
Biology Area | Diabetes associated,Metabolism,Zinc Finger,Fatty acids,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Metabolism,Adipogenesis |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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