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Anti-POU2F3 Polyclonal Antibody

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Catalog No. TMAB-01561
Alias Transcription factor Skn-1, Transcription factor PLA-1, transcription factor 3, POU2F3, POU domain, class 2, transcription factor 3, PLA1, OTF11, Octamer-binding transcription factor 11 (OTF-11), Octamer-binding protein 11 (Oct-11), class 2

Anti-POU2F3 Polyclonal Antibody is a Rabbit antibody targeting POU2F3. Anti-POU2F3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-POU2F3 Polyclonal Antibody

Anti-POU2F3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01561Alias Transcription factor Skn-1, Transcription factor PLA-1, transcription factor 3, POU2F3, POU domain, class 2, transcription factor 3, PLA1, OTF11, Octamer-binding transcription factor 11 (OTF-11), Octamer-binding protein 11 (Oct-11), class 2
Anti-POU2F3 Polyclonal Antibody is a Rabbit antibody targeting POU2F3. Anti-POU2F3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-POU2F3 Polyclonal Antibody is a Rabbit antibody targeting POU2F3. Anti-POU2F3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasTranscription factor Skn-1, Transcription factor PLA-1, transcription factor 3, POU2F3, POU domain, class 2, transcription factor 3, PLA1, OTF11, Octamer-binding transcription factor 11 (OTF-11), Octamer-binding protein 11 (Oct-11), class 2
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (POU2F3) Polyclonal Antibody, Unconjugated (TMAB-01561) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-POU2F3 (TMAB-01561) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 48 kDa
3. Sample:
Testis (Mouse) Lysate at 40 μg
Primary: Anti-POU2F3 (TMAB-01561) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 48 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human POU2F3
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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