Anti-Podoplanin Polyclonal Antibody is a Rabbit antibody targeting Podoplanin. Anti-Podoplanin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat

1. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Podoplanin Protein/gp36 Polyclonal Antibody, Unconjugated (TMAB-01557) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated (TMAB-01557) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Podoplanin (TMAB-01557) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 37 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse Podoplanin

Mouse

PDPN

Podoplanin

Other Cell Types,Endothelium,Blood,Endothelial Cell Markers

May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.