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Anti-Podocin Polyclonal Antibody (Synonyms: Podocin, NPHS2)

Catalog No. TMAB-01556 Copy Product Info
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Anti-Podocin Polyclonal Antibody is a Rabbit antibody targeting Podocin. Anti-Podocin Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

Anti-Podocin Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-01556
Synonyms Podocin, NPHS2

Anti-Podocin Polyclonal Antibody is a Rabbit antibody targeting Podocin. Anti-Podocin Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

Anti-Podocin Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Podocin Polyclonal Antibody is a Rabbit antibody targeting Podocin. Anti-Podocin Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
SynonymsPodocin, NPHS2
Ig Type
IgG
Reactivity
Human,Rat
Verified Activity
1. 25 μg total protein per Lane of various lysates probed with NPHS2 polyclonal antibody, unconjugated (TMAB-01556) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Polyclonal Antibody, Unconjugated (TMAB-01556) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Polyclonal Antibody, Unconjugated (TMAB-01556) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
verifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Peripheral membrane protein.
Tissue SpecificityAlmost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundPodocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NPHS2/Podocin
Antigen Species
Human
Gene Name
NPHS2
Gene ID
Protein Name
Podocin
Uniprot ID
Biology Area
Other Antibodies,Transmembrane,Other Cell Types
Chemical Properties
Molecular WeightTheoretical: 42 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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