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Anti-PLVAP Polyclonal Antibody is a Rabbit antibody targeting PLVAP. Anti-PLVAP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-PLVAP Polyclonal Antibody is a Rabbit antibody targeting PLVAP. Anti-PLVAP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | PV1, FELS |
Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Sheep) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (TMAB-01552) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (TMAB-01552) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining. 3. Sample: Lane 1: Mouse Prostate tissue lysates Lane 2: Mouse Large intestine tissue lysates Primary: Anti-PLVAP (TMAB-01552) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 62 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | PLVAP (Plasmalemma Vesicle Associated Protein) is a Protein Coding gene. Diseases associated with PLVAP include Breast Benign Neoplasm and Thoracic Benign Neoplasm. GO annotations related to this gene include protein homodimerization activity. |
Immunogen | KLH conjugated synthetic peptide: human PLVAP |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Endothelial,Caveolae and Clathrin |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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