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Anti-PLVAP Polyclonal Antibody

😃Good
Catalog No. TMAB-01552
Alias PV1, FELS

Anti-PLVAP Polyclonal Antibody is a Rabbit antibody targeting PLVAP. Anti-PLVAP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PLVAP Polyclonal Antibody

Anti-PLVAP Polyclonal Antibody

😃Good
Catalog No. TMAB-01552Alias PV1, FELS
Anti-PLVAP Polyclonal Antibody is a Rabbit antibody targeting PLVAP. Anti-PLVAP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-PLVAP Polyclonal Antibody is a Rabbit antibody targeting PLVAP. Anti-PLVAP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasPV1, FELS
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (TMAB-01552) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (TMAB-01552) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
3. Sample:
Lane 1: Mouse Prostate tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Primary: Anti-PLVAP (TMAB-01552) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundPLVAP (Plasmalemma Vesicle Associated Protein) is a Protein Coding gene. Diseases associated with PLVAP include Breast Benign Neoplasm and Thoracic Benign Neoplasm. GO annotations related to this gene include protein homodimerization activity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PLVAP
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Endothelial,Caveolae and Clathrin
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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