Anti-PLIN3 Antibody (6Z838) is a Mouse antibody targeting PLIN3. Anti-PLIN3 Antibody (6Z838) can be used in WB,FCM.

IgG2a, κ

6Z838

Human

1. Blank control: HUVEC.
Primary Antibody (green line): Mouse Anti-PLIN3 antibody (TMAB-11554)
Dilution: 1: 25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody: Goat anti-mouse IgG-AF488
Dilution: 1: 200
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Hela cell lysates
Lane 2: K562 cell lysates
Lane 3: Daudi cell lysates
Primary: Anti-PLIN3 (TMAB-11554) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

WB: 1:500-2000; FCM: 1:20-50

Monoclonal

KLH conjugated synthetic peptide: human PLIN3

Human

PLIN3

Perilipin-3