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Anti-PLIN2 Antibody (8I646) is a Rabbit antibody targeting PLIN2. Anti-PLIN2 Antibody (8I646) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
| Description | Anti-PLIN2 Antibody (8I646) is a Rabbit antibody targeting PLIN2. Anti-PLIN2 Antibody (8I646) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Clone | 8I646 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Mouse Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated (TMAB-11553) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 12. 25 ug total protein per lane of various lysates (see on figure) probed with PLIN2 monoclonal antibody, unconjugated (TMAB-11553) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Peripheral membrane protein. |
| Tissue Specificity | Milk lipid globules. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
| Immunogen | A synthesized peptide: human Perilipin 2 |
| Antigen Species | Human |
| Gene Name | PLIN2 |
| Gene ID | |
| Protein Name | Perilipin-2 |
| Uniprot ID | |
| Function | May be involved in development and maintenance of adipose tissue. |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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