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Anti-Plakophilin 2 Polyclonal Antibody
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Catalog No. TMAB-11521
Anti-Plakophilin 2 Polyclonal Antibody is a Rabbit antibody targeting Plakophilin 2. Anti-Plakophilin 2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Plakophilin 2 Polyclonal Antibody
Anti-Plakophilin 2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11521
Anti-Plakophilin 2 Polyclonal Antibody is a Rabbit antibody targeting Plakophilin 2. Anti-Plakophilin 2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-Plakophilin 2 Polyclonal Antibody is a Rabbit antibody targeting Plakophilin 2. Anti-Plakophilin 2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Plakophilin 2 Polyclonal Antibody, Unconjugated (TMAB-11521) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cell junction, desmosome.Note=Nuclear and associated with desmosomes. |
| Tissue Specificity | Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains, and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin-1 mediates increases in desmosomal protein content, desmosome assembly, and regulation of cell migration. Plakophilin-2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin-3 plays a role in both desmosome-dependent adhesion and signaling pathways. Plakophilin-4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function. |
| Immunogen | KLH conjugated synthetic peptide: human Plakophilin 2 |
| Antigen Species | Human |
| Gene Name | PKP2 |
| Gene ID | |
| Protein Name | Plakophilin-2 |
| Uniprot ID | |
| Function | May play a role in junctional plaques. |
| Molecular Weight | Theoretical: 97 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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