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Anti-PKM2 Antibody (5U320)
😃Good
Catalog No. TMAB-11500
Anti-PKM2 Antibody (5U320) is a Rabbit antibody targeting PKM2. Anti-PKM2 Antibody (5U320) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-PKM2 Antibody (5U320)
Anti-PKM2 Antibody (5U320)
😃Good
Catalog No. TMAB-11500
Anti-PKM2 Antibody (5U320) is a Rabbit antibody targeting PKM2. Anti-PKM2 Antibody (5U320) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PKM2 Antibody (5U320) is a Rabbit antibody targeting PKM2. Anti-PKM2 Antibody (5U320) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Clone | 5U320 |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. |
| Tissue Specificity | Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. |
| Immunogen | A synthesized peptide: human PKM |
| Antigen Species | Human |
| Gene Name | PKM |
| Gene ID | |
| Protein Name | Pyruvate kinase PKM |
| Uniprot ID | |
| Function | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. |
| Molecular Weight | Theoretical: 58 kDa. Actual: 58 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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