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Anti-PKCE Antibody (5H978)

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Catalog No. TMAC-03317
Alias Protein Kinase C ε Type, Protein Kinase C Epsilon Type, PRKCE, PKCE, nPKC-ε, nPKC-Epsilon

Anti-PKCE Antibody (5H978) is a Rabbit antibody targeting PKCE. Anti-PKCE Antibody (5H978) can be used in WB,ICC,IHC,FCM.

Anti-PKCE Antibody (5H978)

Anti-PKCE Antibody (5H978)

😃Good
Catalog No. TMAC-03317Alias Protein Kinase C ε Type, Protein Kinase C Epsilon Type, PRKCE, PKCE, nPKC-ε, nPKC-Epsilon
Anti-PKCE Antibody (5H978) is a Rabbit antibody targeting PKCE. Anti-PKCE Antibody (5H978) can be used in WB,ICC,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-PKCE Antibody (5H978) is a Rabbit antibody targeting PKCE. Anti-PKCE Antibody (5H978) can be used in WB,ICC,IHC,FCM.
SynonymsProtein Kinase C ε Type, Protein Kinase C Epsilon Type, PRKCE, PKCE, nPKC-ε, nPKC-Epsilon
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1,000 dilution. Positive control: Lane1: Jurkat, Lane2: MCF-7, Lane3: HT-29.
2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
6. ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of SH-SY5Y cells with PKC epsilon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 84 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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