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Anti-PKA C alpha+beta Polyclonal Antibody
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Catalog No. TMAB-11475
Anti-PKA C alpha+beta Polyclonal Antibody is a Rabbit antibody targeting PKA C alpha+beta. Anti-PKA C alpha+beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PKA C alpha+beta Polyclonal Antibody
Anti-PKA C alpha+beta Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11475
Anti-PKA C alpha+beta Polyclonal Antibody is a Rabbit antibody targeting PKA C alpha+beta. Anti-PKA C alpha+beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-PKA C alpha+beta Polyclonal Antibody is a Rabbit antibody targeting PKA C alpha+beta. Anti-PKA C alpha+beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PKA C alpha+beta) Polyclonal Antibody, Unconjugated (TMAB-11475) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Brain (Rat) Lysate at 40 μg Primary: Anti-PKA C alpha+beta (TMAB-11475) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/50 kD Observed band size: 50 kD 3. Sample: Testis (Mouse) Lysate at 40 μg Testis (Rat) Lysate at 40 μg Primary: Anti-PKA C alpha+beta (TMAB-11475) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/50 kD Observed band size: 50 kD 4. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Rat Heart tissue lysates Lane 5: Human MCF-7 cell lysates Lane 6: Human Hela cell lysates Lane 7: Human Huvec cell lysates Primary: Anti-PKA C alpha+beta (TMAB-11475) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/50 kD kDa Observed band size: 50 kD kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | oplasm. Cell membrane. Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity). |
| Tissue Specificity | Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. |
| Immunogen | KLH conjugated synthetic peptide: human PKA beta |
| Antigen Species | Human |
| Gene Name | PRKACA |
| Gene ID | |
| Protein Name | cAMP-dependent protein kinase catalytic subunit alpha |
| Uniprot ID | |
| Function | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. |
| Molecular Weight | Theoretical: 40/50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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