Anti-Piwil2 Polyclonal Antibody is a Rabbit antibody targeting Piwil2. Anti-Piwil2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,GuineaPig)

1. Blank control: Mouse spleen Cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Piwil2 antibody (TMAB-11473), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Sample:
Testis (Mouse) Lysate at 40 μg
Testis (Rat) Lysate at 40 μg
HepG2 (Human) Cell Lysate at 30 μg
Lovo (Human) Cell Lysate at 30 μg
293T (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Piwil2 (TMAB-11473) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 110 kD

WB: 1:500-2000; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Piwil2/Mili

Human

PIWIL2

Piwi-like protein 2

Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.