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Anti-PIM1 Antibody (6F773) is a Rabbit antibody targeting PIM1. Anti-PIM1 Antibody (6F773) can be used in WB,ICC/IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-PIM1 Antibody (6F773) is a Rabbit antibody targeting PIM1. Anti-PIM1 Antibody (6F773) can be used in WB,ICC/IF,IHC. |
Synonyms | Proviral integration site 2, Proviral integration site 1, Proto-oncogene serine/threonine-protein kinase Pim-1, Proto oncogene serine/threonine protein kinase Pim 1, PIM3, Pim2, PIM1_HUMAN, pim1 kinase 44 kDa isoform, PIM1, pim 1 oncogene (proviral integration site 1), Pim 1 oncogene, pim 1 kinase 44 kDa isoform, Pim 1 kinase, PIM 1, PIM, Oncogene PIM1, Oncogene PIM 1 |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Western blot analysis of PIM1 on different lysates using anti-PIM1 antibody at 1/1,000 dilution. Positive control: Lane 1: BT-20, Lane 2: HepG2, Lane 3: SW480. 2. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin. 8. ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 32 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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