Anti-PIK3R3 Antibody (3T788) is a Rabbit antibody targeting PIK3R3. Anti-PIK3R3 Antibody (3T788) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

3T788

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with PIK3R3 monoclonal antibody, unconjugated (TMAB-11453) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PIK3R3 Monoclonal Antibody, Unconjugated (TMAB-11453) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

A synthesized peptide: human PI3 kinase p55 gamma

Human

PIK3R3

Phosphatidylinositol 3-kinase regulatory subunit gamma

Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.