Anti-PIK3R2 Antibody (6U162) is a Mouse antibody targeting PIK3R2. Anti-PIK3R2 Antibody (6U162) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1, Kappa

6U162

Human,Mouse,Rat

1. Tissue: Mouse colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-11451
2. Tissue: Human colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-11451
3. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition:4°C overnight
Lysate: 1: LnCap, 2: K562, 3: NIH/3T3, 4: BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 85 kDa
Observed MW: 85 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

PIK3R2

Phosphatidylinositol 3-kinase regulatory subunit beta

Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.