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Anti-PIK3R1 Polyclonal Antibody

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Catalog No. TMAB-01540
Alias PtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1

Anti-PIK3R1 Polyclonal Antibody is a Rabbit antibody targeting PIK3R1. Anti-PIK3R1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-PIK3R1 Polyclonal Antibody

Anti-PIK3R1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01540Alias PtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1
Anti-PIK3R1 Polyclonal Antibody is a Rabbit antibody targeting PIK3R1. Anti-PIK3R1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-PIK3R1 Polyclonal Antibody is a Rabbit antibody targeting PIK3R1. Anti-PIK3R1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
AliasPtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse)
Verified Activity
1. Sample: Heart (mouse) Lysate at 40 μg
Primary: Anti-PI3K p85 (TMAB-01540) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 85 kDa
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (TMAB-01540) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Positive control: H9C2 (2% Paraformaldehyde-fixed)
Isotype Control Antibody
Antibody: Rabbit IgG;
Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody: CD28 Rabbit pAb;
Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA
4. Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-PI3 kinase p85 alpha subunit Polyclonal Antibody (TMAB-01540) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.
5. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (TMAB-01540) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (TMAB-01540) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) polyclonal Antibody, Unconjugated (TMAB-01540) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
8. Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PI3K p85) polyclonal Antibody, Unconjugated (TMAB-01540) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PI3 kinase p85 subunit alpha
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Signal transduction,TLR Signaling,Lipid Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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