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Anti-PIK3R1 Polyclonal Antibody is a Rabbit antibody targeting PIK3R1. Anti-PIK3R1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-PIK3R1 Polyclonal Antibody is a Rabbit antibody targeting PIK3R1. Anti-PIK3R1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Alias | PtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse) |
Verified Activity | 1. Sample: Heart (mouse) Lysate at 40 μg Primary: Anti-PI3K p85 (TMAB-01540) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 85 kDa 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (TMAB-01540) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Positive control: H9C2 (2% Paraformaldehyde-fixed) Isotype Control Antibody Antibody: Rabbit IgG; Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA Secondary Antibody Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: CD28 Rabbit pAb; Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA 4. Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-PI3 kinase p85 alpha subunit Polyclonal Antibody (TMAB-01540) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. 5. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (TMAB-01540) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (TMAB-01540) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) polyclonal Antibody, Unconjugated (TMAB-01540) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 8. Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PI3K p85) polyclonal Antibody, Unconjugated (TMAB-01540) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasmic. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
Immunogen | KLH conjugated synthetic peptide: human PI3 kinase p85 subunit alpha |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Signal transduction,TLR Signaling,Lipid Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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