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Anti-PIK3R1 Antibody (1Z225)
😃Good
Catalog No. TMAB-01539
Alias PtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1
Anti-PIK3R1 Antibody (1Z225) is a Mouse antibody targeting PIK3R1. Anti-PIK3R1 Antibody (1Z225) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-PIK3R1 Antibody (1Z225)
Anti-PIK3R1 Antibody (1Z225)
😃Good
Catalog No. TMAB-01539Alias PtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1
Anti-PIK3R1 Antibody (1Z225) is a Mouse antibody targeting PIK3R1. Anti-PIK3R1 Antibody (1Z225) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-PIK3R1 Antibody (1Z225) is a Mouse antibody targeting PIK3R1. Anti-PIK3R1 Antibody (1Z225) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | PtdIns-3-kinase regulatory subunit p85-alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1 |
| Ig Type | IgG |
| Clone | 1Z225 |
| Reactivity | Human,Mouse,Rat (predicted:Pig) |
| Verified Activity | 1. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) monoclonal Antibody, Unconjugated (TMAB-01539) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Sample: Lane 1: Jurkat (Human) Cell Lysate at 30 μg Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 3: Cerebrum (Mouse) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Lane 5: Heart (Mouse) Lysate at 40 μg Lane 6: Heart (Rat) Lysate at 40 μg Lane 7: Skeletal muscle (Mouse) Lysate at 40 μg Lane 8: Skeletal muscle (Rat) Lysate at 40 μg Lane 9: Spleen (Mouse) Lysate at 40 μg Lane 10: MOLT4 (Human) Cell Lysate at 30 μg Primary: Anti-PI 3 Kinase p85 alpha (TMAB-01539) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 85 kDa Observed band size: 85 kDa 3. Sample: BRL-3A Cell (Rat) Lysate at 40 μg Primary: Anti-PI3K p85 (TMAB-01539) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI3K p85) Monoclonal Antibody, Unconjugated (TMAB-01539) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase p85 alpha) Monoclonal Antibody, Unconjugated (TMAB-01539) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasmic. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
| Immunogen | Recombinant Protein: mouse PI3K p85 Protein |
| Antigen Species | Mouse |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Signal transduction,TLR Signaling,Lipid Kinases |
| Molecular Weight | Theoretical: 80 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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