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Anti-PIK3R1 (animal-free) Antibody (5B88)
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Catalog No. TMAB-11444
Anti-PIK3R1 (animal-free) Antibody (5B88) is a Mouse antibody targeting PIK3R1 (animal-free). Anti-PIK3R1 (animal-free) Antibody (5B88) can be used in WB.
Anti-PIK3R1 (animal-free) Antibody (5B88)
Anti-PIK3R1 (animal-free) Antibody (5B88)
Copy Product Info😃Good
Catalog No. TMAB-11444
Anti-PIK3R1 (animal-free) Antibody (5B88) is a Mouse antibody targeting PIK3R1 (animal-free). Anti-PIK3R1 (animal-free) Antibody (5B88) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,057 | 7-10 days | 7-10 days | |
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-PIK3R1 (animal-free) Antibody (5B88) is a Mouse antibody targeting PIK3R1 (animal-free). Anti-PIK3R1 (animal-free) Antibody (5B88) can be used in WB. |
| Ig Type | IgG |
| Clone | 5B88 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Heart tissue lysates Lane 3: Mouse Spleen tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Muscle tissue lysates Lane 6: Rat Heart tissue lysates Lane 7: Rat Spleen tissue lysates Lane 8: Human Jurkat cell lysates Lane 9: Human K562 cell lysates Lane 10: Human Molt-4 cell lysates Lane 11: Human HL60 cell lysates Primary: Anti-PI 3 Kinase p85 alpha (TMAB-11444) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 80 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasmic. |
| Tissue Specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
| Immunogen | Recombinant Protein: mouse PI3K p85 Protein |
| Antigen Species | Mouse |
| Gene Name | PIK3R1 |
| Gene ID | |
| Protein Name | Phosphatidylinositol 3-kinase regulatory subunit alpha |
| Uniprot ID | |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
| Molecular Weight | Theoretical: 80 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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