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Anti-PI3 Kinase Class 3 Polyclonal Antibody is a Rabbit antibody targeting PI3 Kinase Class 3. Anti-PI3 Kinase Class 3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-PI3 Kinase Class 3 Polyclonal Antibody is a Rabbit antibody targeting PI3 Kinase Class 3. Anti-PI3 Kinase Class 3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
Synonyms | Vps34, Vps 34, PtdIns 3 kinase type 3, PK3C3, PIK3C3, PI3K type 3, PI3 kinase type 3, PI3 Kinase p100, phosphoinositide 3 kinase class 3, Phosphatidylinositol 3 kinase p100 subunit, Phosphatidylinositol 3 kinase class 3, Phosphatidylinositol 3 kinase catalytic subunit type 3, MGC61518, hVps34 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (TMAB-01534) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 9. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PI 3 Kinase Class 3 Polyclonal Antibody, Unconjugated (TMAB-01534) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 10. Sample: brain (mouse) Lysate at 40 μg Primary: Anti-PI 3 Kinase Class 3 (TMAB-01534) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kDa Observed band size: 98 kDa |
Application | |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Midbody. Late endosome. |
Tissue Specificity | Ubiquitously expressed, with a highest expression in skeletal muscle. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Research Background | PI 3 Kinase Class 3 is a member of the PI3/PI4-kinase family. It is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes. It is ubiquitously expressed, with a highest expression in skeletal muscle. |
Immunogen | KLH conjugated synthetic peptide: human PIK3C3/PI3 kinase type 3 |
Antigen Species | Human |
Gene Name | PIK3C3 |
Gene ID | |
Protein Name | Phosphatidylinositol 3-kinase catalytic subunit type 3 |
Uniprot ID | |
Biology Area | TLR Signaling,Lipid metabolism,Lipid Kinases |
Function | Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. |
Molecular Weight | Theoretical: 98 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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