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Anti-PI 3 Kinase p55 gamma Antibody (1C282) is a Rabbit antibody targeting PI 3 Kinase p55 gamma. Anti-PI 3 Kinase p55 gamma Antibody (1C282) can be used in WB,ICC/IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-PI 3 Kinase p55 gamma Antibody (1C282) is a Rabbit antibody targeting PI 3 Kinase p55 gamma. Anti-PI 3 Kinase p55 gamma Antibody (1C282) can be used in WB,ICC/IF,IHC. |
Alias | PtdIns-3-kinase regulatory subunit p55-gamma, PtdIns-3-kinase regulatory subunit gamma, PtdIns 3 kinase p85 gamma, Pik3r3, PI3-kinase subunit p55-gamma, PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PI3 kinase p85 subunit gamma, PI 3 Kinase p55 γ, PI 3 Kinase p55 g, Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma), Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma), Phosphoinositide 3 kinase regulatory subunit polypeptide 3, Phosphoinositide 3 kinase regulatory subunit 3 (gamma), Phosphoinositide 3 kinase regulatory subunit 3, Phosphatidylinositol 3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma), Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3, Phosphatidylinositol 3 kinase regulatory subunit gamma, p55PIK, P55G_HUMAN, p55 gamma, p55, OTTHUMP00000009786, OTTHUMP00000009783, FLJ41892, DKFZp686P05226 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of PI 3 Kinase p55 γ on different lysates using anti-PI 3 Kinase p55 γ antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: Jurkat, Lane 3: Mouse testis. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase p55 antibody. Counter stained with hematoxylin. 4. ICC staining PI 3 Kinase p55 γ in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining PI 3 Kinase p55 γ in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-10000; IHC: 1:200-500; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3?? position. PI 3-kinase p55γ (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55γ protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55γ mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55γ have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55γ interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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