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Anti-Phosphotyrosine Antibody (1A392)
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Catalog No. TMAB-11407
Anti-Phosphotyrosine Antibody (1A392) is a Mouse antibody targeting Phosphotyrosine. Anti-Phosphotyrosine Antibody (1A392) can be used in IHC-P,IHC-Fr,IF.
Anti-Phosphotyrosine Antibody (1A392)
Anti-Phosphotyrosine Antibody (1A392)
Copy Product Info😃Good
Catalog No. TMAB-11407
Anti-Phosphotyrosine Antibody (1A392) is a Mouse antibody targeting Phosphotyrosine. Anti-Phosphotyrosine Antibody (1A392) can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,058 | 7-10 days | 7-10 days | |
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phosphotyrosine Antibody (1A392) is a Mouse antibody targeting Phosphotyrosine. Anti-Phosphotyrosine Antibody (1A392) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 1A392 |
| Reactivity | Species independent |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. |
| Immunogen | KLH conjugated Phosphotyrosine |
| Gene ID |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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