Anti-Phospho1 Polyclonal Antibody is a Rabbit antibody targeting Phospho1. Anti-Phospho1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Sample: Hela Cell (Human) Lysate at 40 μg
Primary: Anti-Phospho1 (TMAB-10276) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
2. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho1) Polyclonal Antibody, Unconjugated (TMAB-10276) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Sample: U87Mg (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho1 (TMAB-10276) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 35 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Phospho1

Human

PHOSPHO1

Phosphoethanolamine/phosphocholine phosphatase

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.