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Anti-Phospho-14-3-3 zeta/delta (Ser58) Polyclonal Antibody is a Rabbit antibody targeting Phospho-14-3-3 zeta/delta (Ser58). Anti-Phospho-14-3-3 zeta/delta (Ser58) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $217 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-14-3-3 zeta/delta (Ser58) Polyclonal Antibody is a Rabbit antibody targeting Phospho-14-3-3 zeta/delta (Ser58). Anti-Phospho-14-3-3 zeta/delta (Ser58) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | p-14-3-3 zeta/delta (Ser58), p-14-3-3 zeta/delta (S58), 14-3-3 zeta/delta (p-Ser58), 14-3-3 zeta/delta (p-S58) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from Hela cells untreated or treated with UV using 14-3-3z(Phospho-Ser58) Antibody TMAC-00012. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3z(Phospho-Ser58) Antibody TMAC-00012(left) or the same antibody preincubated with blocking peptide(right). 3. Immunofluorescence staining of methanol-fixed Hela cells using 14-3-3z(Phospho-Ser58) Antibody TMAC-00012. ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta |
| Antigen Species | human |
| Uniprot ID |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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