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Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody
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Catalog No. TMAB-11422
Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZCWCC1 (Ser739). Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody
Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11422
Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZCWCC1 (Ser739). Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZCWCC1 (Ser739). Anti-Phospho-ZCWCC1 (Ser739) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-phospho-ZCWCC1 (Ser739) Polyclonal Antibody, Unconjugated (TMAB-11422) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-ZCWCC1 (Ser739)) Polyclonal Antibody, Unconjugated (TMAB-11422) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: MCF-7 (Human) Cell Lysate at 30 μg k562 (human) Cell Lysate at 30 μg Primary: Anti-phospho-ZCWCC1 (Ser739) (TMAB-11422) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD 4. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-ZCWCC1 (Ser739)) polyclonal Antibody, Unconjugated (TMAB-11422) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm, cytosol. Note=Mainly located in the nucleus. |
| Tissue Specificity | Highly expressed in smooth muscle, pancreas and testis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The CW domain is a structural module found in many vertebrate, parasitic and plant proteins. It consists of a mononuclear four-cysteine zinc-finger domain that plays a role in DNA binding, chromatin methylation and early embryonic development. ZCWCC1 (zinc finger CW-type coiled-coil domain protein 1), also known as MORC2 (MORC family CW-type zinc finger protein 2) or ZCW3, is a 1,032 amino acid protein that contains one CW-type zinc finger domain. ZCWCC1 is located on chromosome 22 and is ubiquitously expressed with highest expression in pancreas, smooth muscle and testis. Expression of ZCWCC1 is upregulated in hypoxia, a pathological condition characterized by an inadequate supply of oxygen in the blood. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human MORC2 around the phosphorylation site of Ser739 |
| Antigen Species | Human |
| Gene Name | MORC2 |
| Gene ID | |
| Protein Name | ATPase MORC2 |
| Uniprot ID | |
| Function | May act as a transcriptional repressor. Down-regulates CA9 expression. |
| Molecular Weight | Theoretical: 114 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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