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Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZAP70 (Tyr319). Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $217 | 7-10 days | 7-10 days | |
| 100 μL | $318 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZAP70 (Tyr319). Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | ZAP70 (p-Y319), ZAP70 (p-Tyr319), p-ZAP70 (Y319), p-ZAP70 (Tyr319) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from Jurkat cells using Zap-70 (Phospho-Tyr319) Antibody TMAC-04316 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1). 2. Immunofluorescence staining of methanol-fixed Hela cells using Zap-70 (Phospho-Tyr319) Antibody TMAC-04316. 3. Western blot analysis of extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), using Zap-70 (Phospho-Tyr319) Antibody TMAC-04316. ![]() ![]() ![]() |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70 |
| Antigen Species | human |
| Uniprot ID |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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