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Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZAP70 (Tyr319). Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody can be used in IF,IHC,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $217 | 7-10 days | |
100 μL | $318 | 7-10 days |
Description | Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ZAP70 (Tyr319). Anti-Phospho-ZAP70 (Tyr319) Polyclonal Antibody can be used in IF,IHC,WB. |
Synonyms | ZAP70 (p-Y319), ZAP70 (p-Tyr319), p-ZAP70 (Y319), p-ZAP70 (Tyr319) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of extracts from Jurkat cells using Zap-70 (Phospho-Tyr319) Antibody TMAC-04316 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1). 2. Immunofluorescence staining of methanol-fixed Hela cells using Zap-70 (Phospho-Tyr319) Antibody TMAC-04316. 3. Western blot analysis of extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), using Zap-70 (Phospho-Tyr319) Antibody TMAC-04316. |
Application | |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70 |
Antigen Species | human |
Uniprot ID |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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