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Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody
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Catalog No. TMAC-04281
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody
😃Good
Catalog No. TMAC-04281
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $318 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:500-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 100 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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