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Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

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Catalog No. TMAC-04281

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

😃Good
Catalog No. TMAC-04281
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$2177-10 days
100 μL$3187-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Application
Recommended Dose
WB: 1:500-1000
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightActual: 100 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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