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Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody (Synonyms: Wee 1 (p-Ser642), Wee 1 (p-S642), p-Wee 1 (Ser642)|...)

Catalog No. TMAC-04281 Copy Product Info
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Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAC-04281
Synonyms Wee 1 (p-Ser642), Wee 1 (p-S642), p-Wee 1 (Ser642), p-Wee 1 (S642)

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.

Anti-Phospho-Wee 1
(Ser642) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2177-10 days7-10 days
100 μL$3187-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.
SynonymsWee 1 (p-Ser642), Wee 1 (p-S642), p-Wee 1 (Ser642), p-Wee 1 (S642)
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody TMAC-04281. The lane on the right is treated with the antigen-specific peptide.
verifiedActivity
Application
Recommended Dose
WB: 1:500-1000
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightActual: 100 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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