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Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody

Catalog No. TMAB-11399 Copy Product Info
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Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TUBB3 (Ser172). Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-11399

Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TUBB3 (Ser172). Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-TUBB3
(Ser172) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TUBB3 (Ser172). Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (phospho-TUBB3 (Ser172)) Polyclonal Antibody, Unconjugated (TMAB-11399) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Sample:
Brain (Mouse) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-p-TUBB3 (Ser172) (TMAB-11399) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/55 kD
Observed band size: 63 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytoskeleton.
Tissue SpecificityExpression is primarily restricted to centraland peripheral nervous system. Greatly increased expression in mostcancerous tissues.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Concentration1mg/ml
Research BackgroundNeuronal Marker Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human TUBB3 around the phosphorylation site of Ser172
Antigen Species
Human
Gene Name
TUBB3
Gene ID
Protein Name
Tubulin beta-3 chain
Uniprot ID
Function
Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance.
Chemical Properties
Molecular WeightTheoretical: 50-55 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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