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Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody
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Catalog No. TMAB-11399
Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TUBB3 (Ser172). Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody
Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11399
Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TUBB3 (Ser172). Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
Chemical Properties
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| Description | Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TUBB3 (Ser172). Anti-Phospho-TUBB3 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (phospho-TUBB3 (Ser172)) Polyclonal Antibody, Unconjugated (TMAB-11399) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Brain (Mouse) Lysate at 40 μg Cerebellum (Mouse) Lysate at 40 μg Primary: Anti-p-TUBB3 (Ser172) (TMAB-11399) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/55 kD Observed band size: 63 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Tissue Specificity | Expression is primarily restricted to centraland peripheral nervous system. Greatly increased expression in mostcancerous tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | Neuronal Marker Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human TUBB3 around the phosphorylation site of Ser172 |
| Antigen Species | Human |
| Gene Name | TUBB3 |
| Gene ID | |
| Protein Name | Tubulin beta-3 chain |
| Uniprot ID | |
| Function | Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance. |
| Molecular Weight | Theoretical: 50-55 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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