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Anti-Phospho-TPH (Ser260) Polyclonal Antibody
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Catalog No. TMAB-11386
Anti-Phospho-TPH (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TPH (Ser260). Anti-Phospho-TPH (Ser260) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-TPH (Ser260) Polyclonal Antibody
Anti-Phospho-TPH (Ser260) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11386
Anti-Phospho-TPH (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TPH (Ser260). Anti-Phospho-TPH (Ser260) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-TPH (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TPH (Ser260). Anti-Phospho-TPH (Ser260) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: HepG2 Cell (Human) Lysate at 40 μg Primary: Anti-phospho-TPH (Ser260) (TMAB-11386) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 49 kD Observed band size: 49 kD 2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TPH (Ser260)) Polyclonal Antibody, Unconjugated (TMAB-11386) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Lane 1: Mouse Cerebellum tissue lysates Lane 2: Rat Cerebellum tissue lysates Primary: Anti-phospho-TPH (Ser260) (TMAB-11386) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 50 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Isoform 2 seems to be less widely expressed than isoform 1. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human TPH around the phosphorylation site of Ser260 |
| Antigen Species | Human |
| Gene Name | TPH1 |
| Gene ID | |
| Protein Name | Tryptophan 5-hydroxylase 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 49 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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