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Anti-Phospho-TPH (Ser260) Polyclonal Antibody

Catalog No. TMAB-11386 Copy Product Info
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Anti-Phospho-TPH (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TPH (Ser260). Anti-Phospho-TPH (Ser260) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-TPH (Ser260) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-11386

Anti-Phospho-TPH (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TPH (Ser260). Anti-Phospho-TPH (Ser260) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-TPH
(Ser260) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-TPH (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TPH (Ser260). Anti-Phospho-TPH (Ser260) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample: HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-TPH (Ser260) (TMAB-11386) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TPH (Ser260)) Polyclonal Antibody, Unconjugated (TMAB-11386) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Primary: Anti-phospho-TPH (Ser260) (TMAB-11386) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityIsoform 2 seems to be less widely expressed than isoform 1.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009].
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human TPH around the phosphorylation site of Ser260
Antigen Species
Human
Gene Name
TPH1
Gene ID
Protein Name
Tryptophan 5-hydroxylase 1
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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