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Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TOB1 (Ser164). Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody can be used in IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $218 | 7-10 days | 7-10 days | |
| 100 μL | $318 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TOB1 (Ser164). Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody can be used in IHC,WB. |
| Synonyms | TOB1 (p-Ser164), TOB1 (p-S164), p-TOB1 (Ser164), p-TOB1 (S164) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody TMAC-04076. The lane on the left is treated with antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody TMAC-04076 (left) or the same antibody preincubated with blocking peptide (right). ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | This gene encodes a member of the tob/btg1 family of proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 40 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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