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Anti-Phospho-TH (Ser31) Polyclonal Antibody
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Catalog No. TMAB-11377
Anti-Phospho-TH (Ser31) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TH (Ser31). Anti-Phospho-TH (Ser31) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-TH (Ser31) Polyclonal Antibody
Anti-Phospho-TH (Ser31) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-11377
Anti-Phospho-TH (Ser31) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TH (Ser31). Anti-Phospho-TH (Ser31) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-TH (Ser31) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TH (Ser31). Anti-Phospho-TH (Ser31) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (TMAB-11377) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Adrenal gland (Mouse) Lysate at 40 μg Lane 2: Adrenal gland (Rat) Lysate at 40 μg Primary: Anti-Phospho-TH (Ser31) (TMAB-11377) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (TMAB-11377) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (TMAB-11377) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (TMAB-11377) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mainly expressed in the brain and adrenal glands. |
| Tissue Specificity | Mainly expressed in the brain and adrenal glands. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | bs-0016P is one synthetic peptide derived from human TH. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthesised phosphopeptide: rat TH around the phosphorylation site of Ser31 |
| Antigen Species | Rat |
| Gene Name | TH |
| Gene ID | |
| Protein Name | Tyrosine 3-monooxygenase |
| Uniprot ID | |
| Function | Plays an important role in the physiology of adrenergic neurons. |
| Molecular Weight | Theoretical: 56 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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